Abstract: Aiming at the problems of insufficient stability, easy deformation and damage, and positioning deviation in the original positioning tooling of vertical machining centers, which uses four independent positioning columns combined with screws for tightening, this paper proposes an optimization scheme for an integrated positioning structure. The improvement scheme utilizes the original positioning column mounting base, adopts interpolation milling technology to process the annular positioning stop, and replaces the discrete positioning column design. At the same time, it retains the four-screw tightening layout and adds an arc transition structure in the workpiece placement area. The optimized tooling significantly improves rigidity through the constraints of the integrated contact surface, avoiding systematic positioning failure caused by local deformation. Practical application shows that this optimization design provides practical reference for high-precision and high-stability positioning tooling design.